At that time, the early signs had been dismissed as particles, however the retrospective analysis demonstrated shared features While using the problematic cells in lifestyle. Tracing the an infection helped the lab create more laboratory techniques for screening and security of assay-significant cell lines.
As samples are obtained about the Attune CytPix Flow Cytometer, the significant-speed brightfield camera captures and shops visuals of detected events at a rate around 6,000 images for each next, with regards to the flow rate and image sizing. For bigger adaptability, Attune Cytometric Software program helps you to change the image seize frequency as essential and choose a certain gate for image capture, if essential.
Blend acoustic focusing technologies with superior-parameter Investigation for flexible spectral unmixing and standard workflows.
The award-profitable Invitrogen Attune CytPix Flow Cytometer combines acoustic focusing with a large-velocity brightfield digital camera for simultaneous higher throughput flow cytometry and higher resolution brightfield imaging.
"An image isn't really fairly really worth a thousand dots, nevertheless it really helps you to see visuals. It is excellent to find out pictures that correlate with staining. For example, AnnexinV-optimistic cells which can be morphologically different than nutritious cells, or CD14+ monocytes that are more substantial plus more textured than CD3+/CD4+/CD14- lymphocytes.
Below, a seasoned user has gated singlets confidently. Soon after assessing the manual singlet gate, the CytPix image-derived parameter ParticleCount reveals this gate consists of over four% aggregates.
Pair your Attune flow cytometer having an autosampler for enhanced workflow efficiency, and add robotic integration for max throughput.
Our scientists will style panels for you personally as over and over you will need—cost-free, no product or service buy wanted.
The Attune NxT cytometer could be the responsible workhorse instrument your lab can depend on for dependable and trusted effectiveness. The Attune CytPix design includes a superior-speed brightfield camera and AI-pushed impression Assessment software package, with recent upgrades which includes equipment learning instruments and an intuitive consumer interface, enabling enhanced data precision, accuracy, and faster insights into sample biology.
End users can watch function visuals in true-time to verify sample high quality, optimize protocols, and report the morphology of each cell inhabitants determined for analysis. Imaging capture also Gains from acoustic focusing, which really helps to placement the cells making sure that a pointy, centered impression is acquired.
Seem waves empower precise cell alignment, resulting in exceptional data high quality and ~10X more rapidly sample flow charges.
We previously demonstrated the strength of imaging Car or truck-T/Ramos cell interactions. Permit’s look at just the populace of best curiosity, the double optimistic situations, to learn more. We could now use prolonged morphological parameters (in this article, circularity and skewness of intensity) to additional examine the functions of these populations and refine gating on these situations, rising information robustness.
Leverage the power of State-of-the-art technological innovation and innovation to accelerate your discoveries. Have confidence in during the Attune Flow Cytometer loved ones to deliver the trustworthiness, speed, and accuracy that established your get the job done apart.
No wash/no lyse leukocyte Examination with imaging. Cells were acquired from 24-hour-aged blood by dilution in one mM EDTA ( Specialist UX/UI enables here people to coach AI models for expanded apps. (A) The “Educate Model” feature provides annotation tools and authentic-time design training accuracy.
At the guts of each Attune Flow Cytometer lies our groundbreaking acoustic concentrating know-how, which makes use of audio waves to meticulously align cells within the flow cell. This impressive solution surpasses traditional hydrodynamic solutions, causing quicker processing speeds, Extraordinary data high quality, and amplified sample throughput.
The Attune Xenith cytometer is a substantial-parameter spectral analyzer, giving equally spectral unmixing and standard flow abilities for detailed and versatile cell Examination.
The Attune CytPix Flow Cytometer lets users to visualize samples through acquisition, confirm the precision of gating techniques, and aid benefits with responsible documentation for publication uses.
What seemed to be a homogeneous populace is actually extra numerous—an Perception that needs to be viewed as when interpreting effects.
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